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A 50- L aliquot of plasma and 50 L of internal standard solution (100 g/L Tadalafil-d3 in 50% acetonitrile; Toronto Research Chemicals Inc, Toronto, ON, Canada) were added to 400 L acetonitrile. The mixture was vortexed for 5 sec and then centrifuged approximately at 14,500 g for 5 min at 4 C. A 5- L aliquot of the diluted supernatant A clinical pharmacological approach to the effective and safe use of tadalafil. The mechanism of action leading to penile erection involves inhibition of PDE5, the major cyclic guanosine monophospate (cGMP) hydrolyzing enzyme located in the vascular smooth muscle cells of corpus cavernosum.

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